ASTM E1397-1991(2003) 实验室鼠肝细胞DNA修复化验的标准实施规程

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【英文标准名称】:StandardPracticefortheinvitroRatHepatocyteDNARepairAssay
【原文标准名称】:实验室鼠肝细胞DNA修复化验的标准实施规程
【标准号】:ASTME1397-1991(2003)
【标准状态】:现行
【国别】:
【发布日期】:1991
【实施或试行日期】:
【发布单位】:美国材料与试验协会(ASTM)
【起草单位】:F04.16
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:遗传工程
【英文主题词】:DNA;geneticexamination;hepatocyteDNArepairassay;rats;thymidine;in-vitrorathepatocyteDNArepairassay,practice;
【摘要】:MeasurementofchemicallyinducedDNArepairisameansofassessingtheabilityofachemicaltoreachandaltertheDNA.DNArepairisanenzymaticprocessthatinvolvestherecognitionandexcisionofDNA-chemicaladductfollowedbyDNAstrandpolymerizationandligationtorestoretheoriginalprimarystructureoftheDNA(7).ThisprocesscanbequantitatedbymeasuringtheamountoflabeledthymidineincorporatedintothenuclearDNAofcellsthatarenotinS-phaseandisoftencalledunscheduledDNAsynthesis(UDS)(8).NumerousassayshavebeendevelopedforthemeasurementofchemicallyinducedDNArepairinvariouscelllinesandprimarycellculturesfrombothrodentandhumanorigin(9).TheprimaryrathepatocyteDNArepairassaydevelopedbyWilliams(10)hasproventobeparticularlyvaluableinassessingthegenotoxicactivityandpotentialcarcinogenicityofchemicals(11),(12).Genotoxicactivityisoftenproducedbyreactivemetabolitesofachemical.Theinvitrorathepatocyteassayprovidesasysteminwhichametabolicallycompetentcellisitselfthetargetcellformeasuredgenotoxicity.Mostothershort-termtestsforgenotoxicityemployaratliverhomogenate(S-9)formetabolicactivation,whichdiffersmarkedlyinmanyimportantwaysfromthepatternsofactivationanddetoxificationthatactuallyoccurinhepatocytes.AnextensiveliteratureisavailableontheuseofinvitrohepatocyteDNArepairassays(2,3,6,13-28).1.1ThispracticecoversatypicalprocedureandguidelinesforconductingtheratinvitrohepatocyteDNArepairassay.Theprocedurespresentedherearebasedonsimilarprotocolsthathavebeenshowntobereliable(1-6).1.2Mentionoftradenamesorcommercialproductsaremeantonlyasexamplesandnotasendorsements.Othersuppliersormanufacturersofequivalentproductsareacceptable.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcernsassociatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:A40
【国际标准分类号】:07_080
【页数】:8P.;A4
【正文语种】: